제목 공동연구논문 The Journal of Neuroscience에 게재!!
글쓴이 관리자 작성일 2018-05-26 22:22:32
2018-06-05 05:59:54

KAIST 생명과학과 김은준 교수 연구팀과의 공동연구로 The Journal of Neuroscience (IF: 5.988)에 논문 게재 (2018.05.24). 우리 연구실의 김수정 박사도 공동저자로 참여.

 2018 May 24. pii: 3321-17. doi: 10.1523/JNEUROSCI.3321-17.2018. [Epub ahead of print]

Lrfn2-mutant mice display suppressed synaptic plasticity and inhibitory synapse development and abnormal social communication and startle response.

Li Y1Kim R2Cho YS2,3Song WS4Kim D1Kim K3Roh JD3Chung C1Park H5Yang E6Kim SJ7Ko J7Kim H6Kim MH4Bae YC2Kim E8,3.


SALM1, also known as LRFN2, is a PSD-95-interacting synaptic adhesion molecule implicated in the regulation of NMDA receptor (NMDAR) clustering largely based on in vitro data, although its in vivo functions remain unclear. Here, we found that mice lacking SALM1/LRFN2 (Lrfn2-/- mice) show a normal density of excitatory synapses but altered excitatory synaptic function, including enhanced NMDAR-dependent synaptic transmission but suppressed NMDAR-dependent synaptic plasticity in the hippocampal CA1 region. Unexpectedly, SALM1 expression is detected in both glutamatergic and GABAergic neurons, and Lrfn2-/- CA1 pyramidal neurons show decreases in the density of inhibitory synapses and frequency of spontaneous inhibitory synaptic transmission. Behaviorally, ultrasonic vocalization was suppressed in Lrfn2-/- pups separated from their mothers, and acoustic startle was enhanced, but locomotion, anxiety-like behavior, social interaction, repetitive behaviors, and learning and memory were largely normal in adult male Lrfn2-/- mice. These results suggest that SALM1/LRFN2 regulates excitatory synapse function, inhibitory synapse development, and social communication and startle behaviors in mice.SIGNIFICANCE STATEMENTSynaptic adhesion molecules regulate synapse development and function, which govern neural circuit and brain functions. The SALM/LRFN family of synaptic adhesion proteins consists of five known members whose in vivo functions are largely unknown. Here we characterized mice lacking SALM1/LRFN2 (SALM1 knockout) known to associate with NMDA receptors and found that these mice showed altered NMDA receptor-dependent synaptic transmission and plasticity, as expected, but unexpectedly also exhibited suppressed inhibitory synapse development and synaptic transmission. Behaviorally, SALM1 knockout pups showed suppressed ultrasonic vocalization upon separation from their mothers, and SALM1 knockout adults showed enhanced responses to loud acoustic stimuli. These results suggest that SALM1/LRFN2 regulates excitatory synapse function, inhibitory synapse development, social communication, and acoustic startle behavior.

이전글 고재원 교수님 관련 기사, '한림원의 창' 2018 봄 호에 소개
다음글 고재원 교수님, 3대 과학기술 3대 한림원 공동토론회 참석