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제목 공동연구 논문 "Chemical Communications" 게재
글쓴이 관리자 작성일 2020-01-17 02:23:48
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2020-01-17 02:23:48

포항공대/IBS 김기문 교수, 박경민 박사 및 서울대 이현우 교수 연구진과의 공동연구로 Chemical Communications (IF: 6.164)에 논문 게재 (2020.01.13).


 2020 Jan 13. doi: 10.1039/c9cc09699j. [Epub ahead of print]

Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid.

Sung G1Lee SY2Kang MG3Kim KL4An J5Sim J6Kim S7Kim S8Ko J8Rhee HW2Park KM4Kim K9.

Abstract

In accordance with the rapid increase in demand for selective and spatial chemical tagging, and accurate detection of proteins of interest, we develop a sensitive protein detection method, termed "Supra-blot" capitalizing on high-affinity host-guest interaction between cucurbit[7]uril (CB[7]) and adamantylammonium (AdA). The method can directly detect chemically tagged proteins without false-positive signals caused by endogenous biomolecules. Not only a single specific protein, but also spatially localized proteins in cells were labeled with AdA, and selectively detected by a host molecule-enzyme hybrid, CB[7]-conjugated horseradish peroxidase (CB[7]-HRP) generating amplified chemiluminescence signals. This study shows the great potential of Supra-blot for accurate and reliable detection of proteins of interest in cells.






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